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Promega liposomal reagent transfast
Liposomal Reagent Transfast, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transfast+liposome/liposomal+reagent+transfasttm/pm33475730-39-13-16
Average 90 stars, based on 1 article reviews
liposomal reagent transfast - by Bioz Stars, 2026-11
90/100 stars

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Transfection:

Article Title: Characterization of a nucleopolyhedrovirus with a deletion of the baculovirus core gene Bm67.
Article Snippet: .. BmN cells (26106) were transfected with 2.0 mg DNA extracted from each bacmid by using a TransFast liposome (Promega) or infected with BV at an m.o.i. of 5. ..

Article Title: Cysteine-rich 61 (CCN1) enhances chemotactic migration, transendothelial cell migration, and intravasation by concomitantly up-regulating chemokine receptor 1 and 2.
Article Snippet: .. The CYR61-sense expression constructs were transfected into AGS cells or CYR61-antisense constructs into MKN45 cells using TransFast liposome (Promega). ..

Article Title: Formation of smooth muscle alpha actin filaments in CD34+ bone marrow cells on arterial elastic laminae: potential role of SH2 domain-containing protein tyrosine phosphatase-1.
Article Snippet: Arterial smooth muscle cells (SMCs) are present in the elastic lamina-containing media, suggesting that the elastic laminae may regulate the development of SMCs.. Here, we investigated the role of elastic laminae in regulating the formation of SMα actin filaments inmouseCD34+ bonemarrow cells and the role of a protein tyrosine phosphatase, SH2 domain-containing protein tyrosine phosphatase (SHP)-1, in the mediation of this process.Mouse CD34+ bone marrow cells were isolated by magnetic separation and used for assessing the influence of elastic laminae and collagen matrix on the formation of SM α actin filaments.. CD34+ cells with transgenic SHP-1 knockout or siRNA-mediated SHP-1 knockdown were used to assess the role of SHP-1 inmediating the formation of SM α actin filaments.

Article Title: Characterization of an early gene orf122 from Bombyx mori nucleopolyhedrovirus.
Article Snippet: The open reading frame 122 of Bombyx mori nucleopolyhedrovirus (BmNPV) (Bm122) has been observed to be a conserved gene in the lepidopteran baculoviruses that have been completely sequenced so far.. Its transcript was detected at 3 h post infection (h p.i.) and remained detectable at up to 96 h p.i.. Temporal transcription analysis indicated that Bm122 is transcribed by host RNA polymerase.

Article Title: Characterization of a late gene, ORF75 from Bombyx mori nucleopolyhedrovirus.
Article Snippet: Open reading frame 75 (Bm-p33) of Bombyx mori nucleopolyhedrovirus (BmNPV) is a homologue of Autographa californica multiple nucleopolyhedrovirus ORF92.. The gene is conserved among all baculoviruses that have been completely sequenced to date and is considered to be a baculovirus core set gene.. No amino acid mutation was found in Bm-p33 sequences among six BmNPV strains differing in geography, phenotype, or host.

Infection:

Article Title: Characterization of a nucleopolyhedrovirus with a deletion of the baculovirus core gene Bm67.
Article Snippet: .. BmN cells (26106) were transfected with 2.0 mg DNA extracted from each bacmid by using a TransFast liposome (Promega) or infected with BV at an m.o.i. of 5. ..

Expressing:

Article Title: Cysteine-rich 61 (CCN1) enhances chemotactic migration, transendothelial cell migration, and intravasation by concomitantly up-regulating chemokine receptor 1 and 2.
Article Snippet: .. The CYR61-sense expression constructs were transfected into AGS cells or CYR61-antisense constructs into MKN45 cells using TransFast liposome (Promega). ..

Construct:

Article Title: Cysteine-rich 61 (CCN1) enhances chemotactic migration, transendothelial cell migration, and intravasation by concomitantly up-regulating chemokine receptor 1 and 2.
Article Snippet: .. The CYR61-sense expression constructs were transfected into AGS cells or CYR61-antisense constructs into MKN45 cells using TransFast liposome (Promega). ..

Recombinant:

Article Title: Involvement of Bombyx mori nucleopolyhedrovirus ORF41 (Bm41) in BV production and ODV envelopment.
Article Snippet: The correct recombinant products, vBm, vBmDe and vBmRe bacmid DNAs, were extracted and purified with a QIAGEN large-construct kit and quantified by optical density. .. Analysis of disrupted, repaired, and wt BmNPV replication in BmN cells The recombinant viruses were obtained by transfecting BmN cells (2×106) with 2 μg of vBm, vBmDe and vBmRe each using a TransFast liposome (Promega). ..

Isolation:

Article Title: Formation of smooth muscle alpha actin filaments in CD34+ bone marrow cells on arterial elastic laminae: potential role of SH2 domain-containing protein tyrosine phosphatase-1.
Article Snippet: Arterial smooth muscle cells (SMCs) are present in the elastic lamina-containing media, suggesting that the elastic laminae may regulate the development of SMCs.. Here, we investigated the role of elastic laminae in regulating the formation of SMα actin filaments inmouseCD34+ bonemarrow cells and the role of a protein tyrosine phosphatase, SH2 domain-containing protein tyrosine phosphatase (SHP)-1, in the mediation of this process.Mouse CD34+ bone marrow cells were isolated by magnetic separation and used for assessing the influence of elastic laminae and collagen matrix on the formation of SM α actin filaments.. CD34+ cells with transgenic SHP-1 knockout or siRNA-mediated SHP-1 knockdown were used to assess the role of SHP-1 inmediating the formation of SM α actin filaments.

Incubation:

Article Title: Formation of smooth muscle alpha actin filaments in CD34+ bone marrow cells on arterial elastic laminae: potential role of SH2 domain-containing protein tyrosine phosphatase-1.
Article Snippet: Arterial smooth muscle cells (SMCs) are present in the elastic lamina-containing media, suggesting that the elastic laminae may regulate the development of SMCs.. Here, we investigated the role of elastic laminae in regulating the formation of SMα actin filaments inmouseCD34+ bonemarrow cells and the role of a protein tyrosine phosphatase, SH2 domain-containing protein tyrosine phosphatase (SHP)-1, in the mediation of this process.Mouse CD34+ bone marrow cells were isolated by magnetic separation and used for assessing the influence of elastic laminae and collagen matrix on the formation of SM α actin filaments.. CD34+ cells with transgenic SHP-1 knockout or siRNA-mediated SHP-1 knockdown were used to assess the role of SHP-1 inmediating the formation of SM α actin filaments.

Cell Culture:

Article Title: Formation of smooth muscle alpha actin filaments in CD34+ bone marrow cells on arterial elastic laminae: potential role of SH2 domain-containing protein tyrosine phosphatase-1.
Article Snippet: Arterial smooth muscle cells (SMCs) are present in the elastic lamina-containing media, suggesting that the elastic laminae may regulate the development of SMCs.. Here, we investigated the role of elastic laminae in regulating the formation of SMα actin filaments inmouseCD34+ bonemarrow cells and the role of a protein tyrosine phosphatase, SH2 domain-containing protein tyrosine phosphatase (SHP)-1, in the mediation of this process.Mouse CD34+ bone marrow cells were isolated by magnetic separation and used for assessing the influence of elastic laminae and collagen matrix on the formation of SM α actin filaments.. CD34+ cells with transgenic SHP-1 knockout or siRNA-mediated SHP-1 knockdown were used to assess the role of SHP-1 inmediating the formation of SM α actin filaments.



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